Journal: Journal of pharmacological sciences

Article Title: The effects of Zanthoxylum bungeanum extract on lipid metabolism induced by sterols.

doi: 10.1016/j.jphs.2014.12.002

Figure Lengend Snippet: Fig. 3. Effects of ZBBu on apoB and apoA1 secretion. HepG2 cells were pretreated with 0.05 mg/mle0.2 mg/ml ZBBu for 12 h and stimulated with sterols for 24 h. After the treatment, apoB and apoA1 in supernatants were evaluated by ELISA. ZBBu could reverse the increased level of apoB induced by 25OH þ cho (A). And ZBBu promoted the secretion of ApoA1 (B). Data are expressed as ratios to the control without treatment of sterols and presented as means ± SD. The mean of at least 3 independent experiments is shown. $P < 0.05, $$P < 0.01 versus normal group. *P < 0.05, **P < 0.01 versus control group treated with 25OH þ cho. #P < 0.05 compared these two control groups. Ctrl: control. Sim: simvastatin. 25OH: 25-hydroxycholesterol. Cho: cholesterol.

Article Snippet: Human apoB and apoA1 ELISA kit were purchased from Assaypro (MO, USA) and Shanghai Hufeng Biotechnology Company (Shanghai, China) respectively.

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Journal of Sport and Health Science

Article Title: Exercise promotes angiogenesis by enhancing endothelial cell fatty acid utilization via liver-derived extracellular vesicle miR-122-5p

doi: 10.1016/j.jshs.2021.09.009

Figure Lengend Snippet: Exercise-upregulated miR-122-5p was shuttled by extracellular vesicles and derived from the liver. (A) Electron microscopy analysis revealed typical rounded particles (extracellular vesicle, EV) in isolated fractions; (B) Nanoparticle tracking analysis and Western blot analysis of extracellular vesicles isolated from sedentary and exercised mice; (C) Particle concentration in plasma from sedentary and exercised mice; (D) Contents of miR-122-5p in the extracellular vesicle fraction and the supernatant fraction (extracellular vesicle free) isolated from plasma of sedentary and exercised mice; (E) Expression profile of miR-122-5p in different tissues in response to exercise; (F) Expression profile of pre-miR-122-5p in different tissues in response to exercise; (G) Liver-specific knockdown of miR-122-5p by AAV8 carrying antisense miR-122-5p oligomers was used to test whether liver-derived miR-122-5p contributes to exercise-induced angiogenesis; (H) AAV infection downregulated miR-122-5p in the liver, skeletal muscle, heart, and kidney in sedentary mice and blocked the upregulation of miR-122-5p in the liver and skeletal muscle in response to exercise; (I) Liver-specific knockdown of miR-122-5p abolished the effects of exercise in upregulation of miR-122-5p in plasma; (J and K) Liver-specific knockdown of miR-122-5p abolished the effects of exercise in promotion of muscle performance as detected by (J) forelimb grip strength and (K) motor coordination; (L) AAV-anti-122 blocked the upregulation of serum VEGF concentration induced by exercise; (M and N) Liver-specific knockdown of miR-122-5p abolished the effects of exercise in promotion of angiogenesis as detected by (M) CD31 staining and (N) expression levels of CD31 and VEGF in skeletal muscles. Scale bars: 100 nm (A); 100 μm (M). n = 6. Values are presented as mean ± SEM. Data are analyzed using the unpaired Student t test (C‒F,) and two-way ANOVA followed by unpaired t tests (H‒N). * p < 0.05; ** p < 0.01. AAV = adeno-associated virus; ANOVA = analysis of variance; APOA1 = apolipoprotein A1; CD31 = platelet/endothelial cell adhesion molecule-1; DAPI = 4’,6-diamidino-2-phenylindole; Exe = exercise; GM130 = golgi matrix protein 130; NC = negative control; Sed = sedentary; TSG101 = tumor susceptibility 101; VEGF = vascular endothelial growth factor.

Article Snippet: CD36 (18836), CPT1A (15184), carnitine palmitoyl-transferase 1B (CPT1B; 22170), VEGF (19003), apolipoprotein A1 (APOA1; 14427), and golgi matrix protein 130 (GM130; 11308) were purchased from Proteintech.

Techniques: Derivative Assay, Electron Microscopy, Isolation, Western Blot, Concentration Assay, Clinical Proteomics, Expressing, Knockdown, Infection, Staining, Muscles, Virus, Negative Control

Journal: BMC Neurology

Article Title: Exploration of candidate serum biomarkers potentially related to the chronic pain condition in Medication–overuse headache

doi: 10.1186/s12883-019-1469-1

Figure Lengend Snippet: CPT values and serum proteins levels in healthy individuals and MOH patients

Article Snippet: Especially, the expression level of the following proteins was evaluated: L-PGDS (human Prostaglandin D Synthase, Lipocalin-type, ELISA kit, BioVendor, Brno, CZ), VDBP (human Vitamin D-binding protein, ELISA kit, Cusabio Biotech, USA), APOE (human apolipoprotein E, Apo-E, ELISA kit, Cusabio Biotech, USA) and APOA1 (human apolipoprotein A1, Apo-A1, ELISA kit, Cusabio Biotech, USA).

Techniques:

Journal: BMC Neurology

Article Title: Exploration of candidate serum biomarkers potentially related to the chronic pain condition in Medication–overuse headache

doi: 10.1186/s12883-019-1469-1

Figure Lengend Snippet: Serum proteins levels in MOH patients, according to the prevalent acute headache medication

Article Snippet: Especially, the expression level of the following proteins was evaluated: L-PGDS (human Prostaglandin D Synthase, Lipocalin-type, ELISA kit, BioVendor, Brno, CZ), VDBP (human Vitamin D-binding protein, ELISA kit, Cusabio Biotech, USA), APOE (human apolipoprotein E, Apo-E, ELISA kit, Cusabio Biotech, USA) and APOA1 (human apolipoprotein A1, Apo-A1, ELISA kit, Cusabio Biotech, USA).

Techniques: